FastQCFastQC Report
Mon 7 May 2018
OD10_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD10_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103585741
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCCCGAATCTCGTATG5077100.4901350273682939TruSeq Adapter, Index 18 (97% over 37bp)
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG1038160.10022228831669024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT238700.035.1070631
CGCGATC639050.025.9776941
CGACATC385500.025.7395152
CGATCCC744350.022.378673
GCGCGAT492350.022.1457751
CCCACTA935250.018.734087
CACTACT936100.018.2093339
CGGCGAT292450.017.4795931
CCACTAC1049400.016.8604328
CCCTACG398150.016.8249669
TACCCTA429850.016.2541187
ATCCCAC1201250.015.6041025
TTCGCTC319600.015.5797252
TACTCGG913350.015.3545017
ACTACCC527100.014.5521995
TCGCCGA618200.014.0953637
GCACTCG735000.013.9014943
CTTGACT882000.013.764031
CGCCGAA609200.013.7599638
GATCCCA1310550.013.6381394
Produced by FastQC (version 0.11.3)