FastQCFastQC Report
Mon 7 May 2018
OD1_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD1_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88495297
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCCACTATCTCGTATG4013180.4534907657296184TruSeq Adapter, Index 1 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT213650.026.0942541
CGCGATC471650.022.2980581
CGACATC349200.020.0622522
CGGCGAT258600.019.5321461
GCGCGAT355500.018.9169621
CGATCCC586550.018.1182293
TTCGCTC291000.016.2312492
CGGCAAT199950.015.2878681
CACTACT730900.015.0945479
TCGCTCG173950.014.9422923
CGGCGCT620500.014.204351
TACTCGG615700.013.1965587
CCACTAC878900.013.1668688
ATCCCAC968600.012.8022425
CCCTACG340400.012.3046529
TACCCTA365300.012.155467
CGGCACT416600.012.1418421
CCGGAAT225450.011.9122871
ACTACCC451550.011.8261475
CTTGACT745450.011.8039351
Produced by FastQC (version 0.11.3)