FastQCFastQC Report
Mon 7 May 2018
OD1_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD1_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88495297
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3983540.45014143519965816Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT214550.026.4324721
CGCGATC448350.022.5789931
CGACATC365650.020.078342
GCGCGAT336950.018.888451
CGATCCC581450.017.5809633
CGGCGAT257500.017.3468041
CACTACT732000.014.5439999
CCCACTA748650.014.5277437
CGGCAAT199950.014.2725181
TTCGCTC288700.013.8596092
CCACTAC846200.013.4557348
CGGCGCT617700.013.3401771
TACTCGG633500.012.734577
ATCCCAC967350.012.72853855
TACCCTA367650.012.7203057
CCCTACG359450.012.2109499
TCGCTCG180250.012.035993
CTTGACT740400.011.9180491
TTGACTA450950.011.7562952
ACTACCC480350.011.7249145
Produced by FastQC (version 0.11.3)