FastQCFastQC Report
Mon 7 May 2018
OD2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109087956
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATATCATCTCGTATG2910890.2668388066598296TruSeq Adapter, Index 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT240300.023.8069521
CGCGATC460050.023.7312851
GCGCGAT343750.021.6393011
CGGCGAT308300.018.7920681
CGATCCC616500.017.8690813
CGACATC433250.017.533352
TTCGCTC318650.015.0265322
CGGCGCT730350.014.6793471
CCCACTA836550.014.1517587
CGGCAAT277450.014.113431
CACTACT832400.013.9024189
CCACTAC973550.012.995628
TCGCTCG200100.012.7379653
TACCCTA376800.012.4147757
CCGGAAT286700.012.1856461
CCCTACG367700.012.1739959
CGGCACT525350.012.10870551
CCGCGCT1012200.011.7782981
TACTCGG638050.011.7304597
ATCCCAC1138550.011.6940995
Produced by FastQC (version 0.11.3)