FastQCFastQC Report
Mon 7 May 2018
OD2_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD2_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109087956
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2895710.2654472689909049Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC451200.022.4218851
GCGACAT256950.022.214481
GCGCGAT335300.019.6359081
CGGCGAT301850.017.7493671
CGATCCC627500.016.8516543
CGACATC444650.016.5432782
CGGCAAT277700.014.7719711
CGGCGCT731700.014.04583551
CCCACTA827300.013.641567
TTCGCTC322600.013.2492872
CACTACT838100.013.0456969
CGGCACT539100.012.1044811
TACCCTA373350.011.9873377
CCACTAC978100.011.9349598
CTTGACT891000.011.682071
CCGCGCT999750.011.5575961
CCGGAAT292150.011.4679231
CGGCAGT581550.011.4468441
CCCTACG387000.011.3045899
TACTCGG647150.011.2892967
Produced by FastQC (version 0.11.3)