FastQCFastQC Report
Mon 7 May 2018
OD3_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD3_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77849882
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGGTCAAATCTCGTATG5714110.7339908363637596TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT173200.028.4520681
CGCGATC361550.025.2002121
GCGCGAT266400.021.4578151
CGACATC288850.021.0585522
CGATCCC453400.020.164143
CGGCGAT207300.016.5874481
CCCACTA605200.016.0558997
CACTACT587700.016.0441029
CCACTAC679150.014.8269498
CGGCAAT180700.014.6254221
TTCGCTC241800.014.438752
CGGCGCT470200.013.7565351
CGAATCC570350.013.5808943
CCGAAAC330350.013.5028311
GCGACCC525150.013.4848571
TCGCCGA523350.013.381987
GCCGAAT561350.013.3695691
ATCCCAC800500.013.3179325
CGCCGAA514200.013.2701578
GCACTCG620550.013.166693
Produced by FastQC (version 0.11.3)