FastQCFastQC Report
Mon 7 May 2018
OD4_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD4_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111499085
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATACTATCTCGTATG5518580.494943971961743TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT254850.028.7068141
CGCGATC617550.025.8688471
GCGCGAT468450.024.9534151
CGACATC438400.020.8370362
CGATCCC817100.020.0431543
CACTACT956650.017.0719329
CCCACTA981800.017.03057
TTCGCTC355000.015.7962482
CCACTAC1096250.015.6530098
CGGCGAT348050.015.5235841
TACTCGG853850.015.0135567
CGGCAAT260950.014.6334351
TACCCTA393500.014.2858977
ATCCCAC1292650.014.217195
TCGCTCG230950.013.9954933
CGGCGCT772650.013.9477921
CTACTCG960750.013.74766
CCCTACG419950.012.8890679
TCGCTAT152000.012.7873343
CTCGCTA147500.012.78657152
Produced by FastQC (version 0.11.3)