FastQCFastQC Report
Mon 7 May 2018
OD4_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD4_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111499085
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5589870.5013377464039279Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC595200.025.5629521
GCGCGAT453400.023.9998321
GCGACAT273300.023.8784541
CGATCCC808800.019.269233
CGACATC444450.018.416042
CCCACTA966100.016.7724537
CACTACT947700.016.459669
CGGCGAT353900.016.3475341
CCACTAC1105500.014.8788938
TTCGCTC359050.014.5960452
ATCCCAC1276050.013.9679015
CGGCAAT266100.013.7502791
TACTCGG878400.013.6529277
TACCCTA398850.013.2473687
CCGGAAT301450.012.9648581
CGGCGCT803200.012.9543161
CTACTCG980850.012.6386
CCGCGCT1164500.012.3227361
CGGCACT534750.011.99811651
GATCCCA1415950.011.9167364
Produced by FastQC (version 0.11.3)