FastQCFastQC Report
Mon 7 May 2018
OD5_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD5_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106710982
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3715250.34816004223445346Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT254300.024.8463341
CGCGATC496050.023.8071161
GCGCGAT366400.022.0802861
CGACATC412700.018.9133632
CGGCGAT296450.018.0289021
CGATCCC681750.017.848863
CCCACTA855450.015.1310647
CGGCAAT239500.014.6734961
CACTACT872450.014.200599
CGGCGCT721300.013.9060091
CCACTAC1006750.013.3213288
TTCGCTC321650.012.9171352
ATCCCAC1151800.012.2837435
TACTCGG758650.012.1462717
CCGGAAT275450.012.09390451
TCGATCT596400.011.9159423
TACCCTA390550.011.8156017
CGGCACT494450.011.6977581
CGGAATC304950.011.6882772
CTTGACT857000.011.268391
Produced by FastQC (version 0.11.3)