FastQCFastQC Report
Mon 7 May 2018
OD6_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD6_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94727091
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGAATCTCGTATG7091310.7486042192512805TruSeq Adapter, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT216150.022.829031
CGCGATC430400.021.5641351
GCGCGAT329350.019.6976151
CGACATC373900.017.5763452
CGGCGAT279950.017.0560781
CGATCCC563600.016.7307423
TTCGCTC312500.016.722452
CGGCAAT205950.015.4328471
TCGCTCG193050.014.8398033
CGGCGCT649100.013.9408291
CCCACTA733100.013.8438187
CACTACT708550.013.6733389
CCGGAAT262750.013.0080431
CGGCACT447100.012.6272471
CCACTAC841250.012.42342958
TACCCTA332150.012.4171057
CGGCAGT498450.012.1416641
CCCTACG342550.011.8930289
ACTACCC456550.011.8242315
CCGCGCT795700.011.8192361
Produced by FastQC (version 0.11.3)