FastQCFastQC Report
Mon 7 May 2018
OD6_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD6_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94727091
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7024150.7415143783946665Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT225700.022.3880141
CGCGATC420500.021.4557021
GCGCGAT310000.018.4968641
CGACATC388250.017.2852952
CGGCGAT288350.016.6094741
CGATCCC575950.016.2269743
CGGCAAT216750.014.5956241
TTCGCTC308300.013.7566972
CCCACTA735700.013.4449477
CGGCGCT650750.013.2452551
CACTACT729750.013.0709079
TACCCTA346050.012.4196287
CGTATCA1832250.012.03159745-49
CCACTAC862650.011.8580518
CTTGACT755150.011.7535081
TCGCTCG200600.011.7371993
CCGTATC1888450.011.6331345-49
CGGCACT444400.011.6175131
ACTACCC489900.011.5814335
GCCGTAT1944450.011.25440845-49
Produced by FastQC (version 0.11.3)