FastQCFastQC Report
Mon 7 May 2018
OD7_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD7_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88644239
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCCTCATCTCGTATG3713760.41895108378109036TruSeq Adapter, Index 9 (97% over 37bp)
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG904660.10205513750307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT207700.027.2014261
CGCGATC514850.026.6962361
GCGCGAT374200.023.9908221
CGATCCC632900.022.3562873
CGACATC322800.020.9461542
CCCACTA812750.018.0503227
CACTACT801050.017.9005939
CGGCGAT260750.017.2006361
CCACTAC907900.016.5708778
CGGCAAT192150.015.849621
TACTCGG705900.015.6022047
ATCCCAC1042400.015.4892935
TTCGCTC270950.015.1746672
TACCCTA327350.014.2715157
CCCTACG318600.014.0760239
CTACTCG818650.014.0160876
CGGCGCT634500.014.0119121
CCGGAAT219950.013.6161241
GATCCCA1158700.013.2430824
ACTACCC444550.013.0512225
Produced by FastQC (version 0.11.3)