FastQCFastQC Report
Mon 7 May 2018
OD7_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD7_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88644239
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3695070.4168426557308479Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT212850.026.8853661
CGCGATC501600.026.2938141
GCGCGAT359250.024.6993751
CGATCCC637700.020.8541373
CGACATC341050.020.4038412
CCCACTA825650.016.8818027
CACTACT799900.016.839029
CGGCGAT260300.016.185731
TACTCGG729400.015.4789797
CGGCAAT200500.015.4584011
CCACTAC928350.015.2312378
ATCCCAC1028500.014.6233625
TACCCTA330650.014.2111697
CTACTCG826400.013.9675196
CCCTACG339000.013.5839789
TTCGCTC276950.013.511562
CGGCGCT637950.013.4840411
GATCCCA1129350.012.7822944
CCGGAAT228750.012.7485431
TTGACTA436650.012.5910932
Produced by FastQC (version 0.11.3)