FastQCFastQC Report
Mon 7 May 2018
OD8_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD8_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75375882
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTTGTATCTCGTATG9732391.2911809111567012TruSeq Adapter, Index 14 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAT186750.016.9689791
CGGCAAT160250.015.9825991
GCGACAT140150.015.2289911
CGCGATC268650.015.2161161
CGGCGCT445200.015.0974211
TTCGCTC225500.014.5933062
CCGTCTT2078500.014.46948250-54
GCTTGAA2089850.014.32824160-64
CGTCTTC2140800.014.15199150-54
TGCTTGA2208150.013.85949555-59
CTTGAAA2221800.013.79879560-64
GCCGTCT2149600.013.78322150-54
CGACATC233750.013.7393832
GCGCGAT228350.013.5924731
CGGCAGT328500.013.4129451
ATGCCGT2263900.013.32287545-49
TGCCGTC2273300.013.30135845-49
CGGCACT302950.013.2545271
CCGCGCT657200.013.133641
CTGCTTG2478200.012.493955-59
Produced by FastQC (version 0.11.3)