FastQCFastQC Report
Mon 7 May 2018
OD8_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD8_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75375882
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9570511.2697045455468103Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGGT482800.016.9613827
CGGCGAT185250.016.0999741
GCGACAT147700.015.7774041
CGGCAAT159100.015.7062731
CGCGATC249200.014.6443561
CGTATCA2057900.014.47497545-49
CGGCGCT440900.014.1442011
CCGTATC2140450.013.9274845-49
CCGCGCT641450.013.7661711
CGACATC247500.013.7236892
ATCATTA2245850.013.51264650-54
GCCGTAT2210900.013.45634345-49
GTATCAT2180900.013.41883350-54
GCGCGAT205350.013.06096651
CGGCACT302550.012.9340061
TCATTAA2356300.012.93116450-54
TTCGCTC222450.012.9075722
CGGCAGT325250.012.7748171
CGCCGTA2322500.012.76698745-49
CCGGAAT184300.012.7237051
Produced by FastQC (version 0.11.3)