FastQCFastQC Report
Mon 7 May 2018
OD9_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD9_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99448507
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGGACCATCTCGTATG3357360.3375978283917324TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT213900.028.699131
CGACATC333200.022.0055562
CGCGATC462500.020.4175321
CGATCCC513300.018.4198993
GCGCGAT363700.018.1509481
CCCTACG321650.016.7775869
CGGCGAT245050.016.2285521
TTCGCTC310600.016.0080992
CGGCAAT205850.014.577031
TACCCTA395900.014.3765027
CCCACTA722700.014.2078447
CGGCGCT613300.013.8527731
ACTACCC475150.013.4174645
CCGGAAT227200.013.2708751
CACTACT756950.013.1559049
GACTACC456850.012.5844214
CGGCACT421250.012.5301071
TCGCTCG171800.012.31366353
CCACTAC851300.012.3063868
CCGCGCT935300.012.2377551
Produced by FastQC (version 0.11.3)