FastQCFastQC Report
Mon 7 May 2018
OD9_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD9_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99448507
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3295910.33141875121363057Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT222100.028.0525421
CGACATC352200.022.0285512
CGCGATC428550.021.7222121
GCGCGAT330950.018.493791
CGATCCC510350.018.1767163
CGGCGAT252600.017.0045871
CCCTACG323650.014.8608349
CCCACTA718400.014.2217337
CGGCAAT211800.013.8431821
TTCGCTC306550.013.6748032
CCGGAAT224350.013.2321621
CGGCGCT615750.013.1302471
CACTACT753600.013.0317439
TACCCTA390900.012.7649357
CGCGAAT55750.012.6220021
CCACTAC842500.012.5704328
CGAATCC423450.012.1761733
CCGCGCT924200.011.9522361
CGGCACT412200.011.8609561
ACTACCC486800.011.7871965
Produced by FastQC (version 0.11.3)