FastQCFastQC Report
Mon 7 May 2018
YD10_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD10_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85013968
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAACTGATCTCGTATG3845330.45231743564775145TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT196900.026.2549211
CGCGATC398100.023.2107451
GCGCGAT301800.021.058141
CGACATC317350.020.3382362
CGATCCC508050.018.3409863
CGGCGAT245650.017.1594031
CGGCAAT197400.016.3357121
TTCGCTC265950.015.9087722
TCGCTCG162200.014.7724573
CCCACTA684600.014.6830947
CACTACT660300.014.4276249
CGGCGCT583700.013.7493981
TACCCTA308150.013.4265287
CCGGAAT221550.013.3801971
CCACTAC766050.013.2812948
CCCTACG306450.012.7493589
CGGCACT398850.012.7074611
ATCCCAC911500.012.2991545
ACTACCC420550.012.1822315
CTTGACT705900.012.06571
Produced by FastQC (version 0.11.3)