FastQCFastQC Report
Mon 7 May 2018
YD10_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD10_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85013968
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3761480.44245435056036914Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT199050.025.1128251
CGCGATC386550.024.1756361
GCGCGAT282250.021.0600911
CGACATC331800.019.7423252
CGATCCC512350.018.7513833
CGGCGAT247100.017.8565311
CCCACTA691250.014.821897
CACTACT665700.014.7090419
CGGCGCT588300.013.8915071
TTCGCTC275400.013.6550742
TACCCTA316700.013.5534587
CGGCAAT192900.013.4886821
CCACTAC775700.013.3377138
CCCTACG313900.012.8944529
CGGCACT400300.012.8352931
TCGCTCG175000.012.6151943
ATCCCAC906400.012.58975
CCGGAAT231850.012.4555381
CTTGACT694650.011.9863141
CGCGAAT51500.011.95488551
Produced by FastQC (version 0.11.3)