FastQCFastQC Report
Mon 7 May 2018
YD1_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD1_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97801958
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5132210.5247553428327069Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT250950.028.066671
CGCGATC565150.026.1524281
GCGCGAT412400.022.565361
CGACATC416100.021.510922
CGATCCC733350.020.7082213
CCCACTA871750.017.76687
CGGCGAT282950.017.4376411
CACTACT897900.016.8252669
TACCCTA372650.015.981077
CCACTAC1024700.015.5144748
CGGCAAT222550.014.9878491
ATCCCAC1158800.014.7729075
TTCGCTC315750.014.6742192
CCCTACG403050.014.526119
TCGCTCG190700.014.088673
ACTACCC534800.013.714655
GCGATCC1054950.013.7004472
TACTCGG695950.013.4705367
TTGACTA513500.013.3878282
GATCCCA1271600.012.8861834
Produced by FastQC (version 0.11.3)