FastQCFastQC Report
Mon 7 May 2018
YD2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82118504
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCTATCTCGTATG6883540.8382446908677245TruSeq Adapter, Index 13 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT185800.023.9360791
CGCGATC391350.020.8124051
GCGCGAT298800.019.7072221
CGGCGAT243250.018.3727021
CGACATC328900.017.1632542
CGATCCC516200.016.1800373
GAGCACA6935050.015.8564779
AGAGCAC7077350.015.4044518
TCGTATG1510700.014.56528740-44
CGTATGC1499100.014.41779745-49
CGGCAAT195100.014.3635221
CGGCGCT579450.014.2698651
CCGTCTT1553150.014.209064550-54
AAGAGCA7685950.014.1818657
ATGCCGT1567650.014.14092945-49
CGGAAGA7686450.014.1015094
TTCGCTC259550.014.0648262
TCGGAAG7734300.014.0040263
CCCACTA638850.013.9686917
ATCGGAA7829800.013.8325532
Produced by FastQC (version 0.11.3)