FastQCFastQC Report
Mon 7 May 2018
YD2_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD2_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82118504
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6815550.8299651927414556Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT191100.020.894121
CGCGATC376800.020.8642251
GCGCGAT284550.019.1115151
GAGCGTC6079350.017.7214879
AGAGCGT6170250.017.4256788
AAGAGCG6688100.016.0957647
CGGCGAT234450.015.816521
CGGCAAT193500.015.65541651
CGACATC332900.015.6447892
CCGTATC1397300.015.63065945-49
CGTATCA1414500.015.5279545-49
GCCGTAT1402450.015.51076845-49
CGATCCC522200.015.393943
CGCCGTA1444950.014.92429245-49
TCGGAAG7462650.014.448253
CGGAAGA7492950.014.3927114
ATCGGAA7567350.014.173942
GATCGGA7725200.013.8863111
GAAGAGC7828000.013.84190756
CCCACTA642150.013.592447
Produced by FastQC (version 0.11.3)