FastQCFastQC Report
Mon 7 May 2018
YD3_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD3_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85695568
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTTGTCATCTCGTATG8738871.0197575211824257TruSeq Adapter, Index 21 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT222700.030.200581
CGCGATC460700.025.377511
CGACATC341500.023.9884452
GCGCGAT348550.021.579831
CGATCCC573750.021.1671033
CCCACTA731250.017.2947527
TACCCTA328150.017.0653677
CACTACT727600.016.7483779
CCCTACG323200.016.43599
CGGCGAT241450.016.1808931
CCACTAC820450.015.5811428
CGGCAAT188950.015.3819751
TCGCTCG166750.014.9375473
ACTACCC432750.014.7883695
ATCCCAC971600.014.5663785
TTCGCTC283400.014.4531642
TTGACTA452300.013.9102642
CTTGACT731550.013.7057371
GACTACC432750.013.3914574
GCGATCC889550.012.9236462
Produced by FastQC (version 0.11.3)