FastQCFastQC Report
Mon 7 May 2018
YD3_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD3_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85695568
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8796701.0265058281660493Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT226050.028.7733021
CGCGATC452700.024.0701561
CGACATC353900.021.6838282
GCGCGAT338400.021.1273421
CGATCCC572150.019.6525693
CCCACTA714250.016.2162697
CGGCAAT188900.015.4885761
CACTACT726050.015.4268799
CGGCGAT248250.015.2711551
TACCCTA341850.014.7192857
CCCTACG345250.014.4280429
CCACTAC837400.014.1495398
ATCCCAC963650.013.5284835
TTGACTA451400.013.316322
GAGCGTC10286300.013.2584699
ACTACCC464850.013.0701215
AGAGCGT10452050.013.0505928
CTTGACT718150.013.018641
CGTATCA2171100.012.850450545-49
CGGCGCT600100.012.6469941
Produced by FastQC (version 0.11.3)