FastQCFastQC Report
Mon 7 May 2018
YD5_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD5_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100553578
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCCGATCTCGTATG10271041.0214494803954166TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT224150.023.5016631
CGCGATC449400.021.5227491
GCGCGAT348100.018.4476031
CGGCGAT268300.017.8494131
CGACATC379800.017.795912
CGATCCC572850.017.0133693
TTCGCTC304100.014.9355942
CGGCAAT234950.014.9166871
CGGCGCT650300.013.9809931
CCCACTA773250.013.6183367
CACTACT785250.013.14440259
CCGGAAT254600.012.7109241
GAGCACA12793900.012.7034189
AGAGCAC13052500.012.3657088
GCTTGAA2675750.012.284265560-64
CCACTAC900150.012.10627658
TCGCTCG179550.011.9465763
CGGCACT460450.011.9135171
TACTCGG583200.011.872917
CCGTCTT2752950.011.87111450-54
Produced by FastQC (version 0.11.3)