FastQCFastQC Report
Mon 7 May 2018
YD5_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD5_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100553578
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10183381.0127317398889575Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT236550.021.8226721
CGCGATC424600.021.4009741
GCGCGAT328300.019.4485551
CGACATC383700.017.6028792
CGGCGAT264950.016.8856981
CGATCCC579450.016.0419523
CGGCGCT664600.013.9260671
GAGCGTC11626550.013.6280949
AGAGCGT11844350.013.3911328
CCCACTA767700.013.3440197
CGGCAAT230450.013.2495491
TTCGCTC306700.013.2180472
AAGAGCG12550450.012.6658037
CGGCACT479400.012.6313331
CGTATCA2598000.012.41348545-49
CACTACT792750.012.24107559
CCGTATC2694050.011.99709945-49
TACTCGG599850.011.8213437
CCGGAAT261250.011.7996061
GCCGTAT2766750.011.6558445-49
Produced by FastQC (version 0.11.3)