FastQCFastQC Report
Mon 7 May 2018
YD6_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD6_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86690308
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGGGGATCTCGTATG6926580.7990028135555823TruSeq Adapter, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT210900.023.873391
CGCGATC384150.020.8496841
GCGCGAT295050.019.6955491
CGACATC355300.018.2737082
CGGCGAT251950.017.3633771
CGATCCC514200.016.0144063
CGGCAAT203850.014.520471
CGGCGCT625400.013.8044891
CCCACTA651950.013.2927637
TACCCTA304550.013.282537
CACTACT642700.013.08099
CCCTACG315550.012.5229679
TACTCGG501600.012.1542737
CGGCACT422400.012.0914941
CCGGAAT234100.012.0553391
ACTACCC435800.011.941375
CCACTAC769950.011.8164668
CCGCGCT772500.011.8144461
CGGCAGT467600.011.7605031
CTTGACT730000.011.7469691
Produced by FastQC (version 0.11.3)