FastQCFastQC Report
Mon 7 May 2018
YD6_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD6_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86690308
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6926260.7989659005479597Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT214800.022.4286141
CGCGATC365700.022.0631471
GCGCGAT275450.019.5103051
CGACATC359350.017.974032
CGGCGAT254150.016.6801241
CGATCCC518750.015.4484773
CGGCAAT209000.015.3790351
CGGCGCT628650.013.2422681
CCCACTA668950.012.9462797
CACTACT653550.012.8324959
TACCCTA319400.012.6444797
CTTGACT706350.011.972171
CCCTACG334850.011.9318189
TACTCGG523000.011.6725887
CCACTAC776200.011.5743318
ACTACCC464300.011.5376785
TTGACTA424600.011.5318672
CCGCGCT766650.011.5080241
CGGCACT431200.011.47833251
CCGGAAT235200.011.3000441
Produced by FastQC (version 0.11.3)