FastQCFastQC Report
Mon 7 May 2018
YD8_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD8_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114705343
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGGTTATCTCGTATG9973320.869473011383611TruSeq Adapter, Index 3 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT263250.025.6126021
CGCGATC546550.022.5551571
GCGCGAT403200.021.1184521
CGACATC465500.019.5063532
CGATCCC728850.017.6253343
CGGCGAT351300.015.67157651
CCCACTA917200.014.7706217
CGGCAAT260150.014.6830481
CACTACT907800.014.5746639
TTCGCTC355000.013.7930792
CGGCGCT805500.013.6695831
CCACTAC1061850.013.3957328
TACCCTA405100.013.3095317
CGGCACT554500.012.9163491
TACTCGG742250.012.7434897
ACTACCC566050.012.4500025
CCCTACG422150.012.3797839
ATCCCAC1223300.012.3750735
TCGCTCG230900.011.91297153
GCTTGAA2680300.011.88481760-64
Produced by FastQC (version 0.11.3)