FastQCFastQC Report
Mon 7 May 2018
YD8_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD8_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114705343
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9899780.863061801750595Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT276100.024.1226651
CGCGATC530200.022.5808411
GCGCGAT392500.020.2356621
CGACATC482250.018.175312
CGGCGAT361550.016.8204461
CGATCCC741850.016.5296573
CGGCAAT268650.014.2367691
CCCACTA934000.013.9294427
CGGCGCT810550.013.9209441
CACTACT915050.013.6741649
TTCGCTC354850.012.6342672
TACCCTA429800.012.6069717
CCACTAC1089750.012.1697218
CGGCACT559450.012.0490931
CTTGACT922000.011.6819191
CCCTACG441150.011.6454959
TACTCGG752500.011.6089897
ATCCCAC1233450.011.5810535
GTTGGGT721250.011.5233397
ACTACCC609750.011.424385
Produced by FastQC (version 0.11.3)