FastQCFastQC Report
Mon 7 May 2018
YD9_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD9_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91724961
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6177620.6734938813438144Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT217300.025.062941
CGCGATC469750.022.8754771
GCGCGAT350200.020.9517781
CGACATC381000.017.9287872
CGATCCC630200.017.5494823
CGGCGAT288300.016.7295481
CCCACTA743000.015.4537557
CACTACT756650.014.9552959
CGGCAAT221650.014.3524131
CCACTAC883900.013.356138
CGGCGCT683400.013.2355841
TACTCGG693750.013.2033587
ATCCCAC1019350.012.3019795
CGGCACT458500.012.2939071
CCGCGCT889450.012.2214441
CTACTCG781900.012.0643026
TACCCTA336900.011.6095627
CTTGACT714250.011.46321
CGTATCA1790300.011.175645-49
GATCCCA1115350.010.9085324
Produced by FastQC (version 0.11.3)