FastQCFastQC Report
Thu 8 Feb 2018
OD11_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD11_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24676060
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG78500.013.2635851
TAGGCCG19400.010.6874651
TGGGGTT168500.010.5126271
TGGGCCG43400.010.2816791
TGGCCGG64450.010.0356981
TGGGGCG45600.09.5879441
AGGGTTT158800.09.5652871
GGGGTTT206400.09.4417972
AGGGGCG46800.09.2457891
TAGCCAG197500.09.1287551
TGGCGGC53950.08.8976791
TGGCCAG309500.08.7452241
AGGGCCG44500.08.6601351
ACGGGGT71750.08.5120861
TAGCCCG12450.08.5077971
GTAGTCC154400.08.466639526
TCCCGTT37000.08.4664561
AGGCCGG86350.08.4300231
TGGCAAA138850.08.3589151
TAGCCGA10100.08.2559851
Produced by FastQC (version 0.11.5)