FastQCFastQC Report
Thu 8 Feb 2018
OD12_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD12_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24002157
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG80100.012.7254691
TAGGCCG21100.011.86337951
TGGGCCG53350.011.2861211
TGGCGAA28400.010.0844751
TGGGGTT168950.09.9307641
TAGCCAG196050.09.7773231
AGGGTTT147350.09.7336311
AGGGGCG60150.09.560321
TAGGGCG10250.09.46046451
AGGGCGG64450.09.4123331
TGGCCGG72050.09.3584751
TGGGGCG52200.09.1586881
TGGCCAG311750.09.1289491
TAGGCGC12400.09.0931881
TGGCGGC66650.09.0678121
AGGCCGG105300.09.0375771
GGGGTTT208450.08.9255822
TGGGGCC145850.08.9060251
TGGGCGG66200.08.68660451
AGGCGCC88700.08.6695921
Produced by FastQC (version 0.11.5)