FastQCFastQC Report
Thu 8 Feb 2018
OD13_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD13_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34334025
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG90900.011.4562111
AGGGGCG134900.010.910991
TGGGGCG116200.010.6882941
TGGGCCG104450.010.61743451
TGGCGGC149150.010.4277051
TGGCGAC44050.010.2340371
AGGGCCG116250.010.1601761
TGGGGTT234750.09.97637751
TGGGCGG128000.09.9318931
AGGGGCC212200.09.761871
TAGGCCG34150.09.4386151
AGGCGCC149600.09.4019071
AGGGCGG142100.09.2953651
AGGGTTT200700.09.2205891
TAGCCAG222600.09.2146571
AGGCGGC150950.09.0938351
AGGGGGG102150.09.0470791
AGGCGAC45900.09.035831
TGGCGAA44700.08.8245681
TGGCCGG117700.08.7136041
Produced by FastQC (version 0.11.5)