FastQCFastQC Report
Thu 8 Feb 2018
OD14_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD14_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54822756
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG182900.013.5404261
TGGGGTT363850.010.1973481
TAGCCAG458350.010.0239181
TAGGCCG41250.09.5688081
TGGGCCG96350.09.4106161
TGGCCAG673050.09.3564651
TGGCCGG136950.09.0417811
AGGGTTT345600.08.9737231
GGGGTTT434100.08.8889092
AGGGCCG97550.08.8324211
AGGGCGG114350.08.8168831
TGGGGCG93800.08.7286591
TACTCGG224800.08.63785337
TGGCAAA314450.08.363551
AGGGGCG105750.08.360831
CTACTCG234450.08.30151136
TGGCGAA62900.08.2833321
TCCCGTT71750.08.2675651
AGGCGCC160950.08.2400541
TGGCGGC117100.08.1282921
Produced by FastQC (version 0.11.5)