FastQCFastQC Report
Thu 8 Feb 2018
OD15_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD15_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25275544
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG6700.015.8033466
ATACGGC8500.012.7217925
TAGCCGG76000.012.3132311
TAGGCCG20850.010.3825191
TGGGCCG49800.010.0522011
TGGCGAA29300.09.9279521
ACGGCGA13200.09.7280757
TGGGGTT167850.09.6458541
TGATACG10900.09.5073023
ACGACGC9250.09.48316241
TGGCCAG312650.09.4482291
TAGCCAG197450.09.2390851
GATACGG11950.09.2374944
AGGGGCG55700.09.1898451
AGGGTTT151850.09.1178231
CACGACG8400.09.10389740
GGGGTTT196050.08.997482
TGGCCGG68650.08.9672581
TGGGGCG48800.08.9643631
TACACGA9900.08.860599538
Produced by FastQC (version 0.11.5)