FastQCFastQC Report
Thu 8 Feb 2018
OD16_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD16_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25604234
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG3858.311508E-89.3636946
TAGCCGG52850.08.7912321
AGGGCCG36100.08.5593511
TGGGCCG33700.08.2319511
TGGGGCG34800.08.1013671
TGGCGGC42450.07.54463341
TGGCCCG27550.07.53171251
TGGCCGG54450.07.331671
ACGACGC5901.3284443E-77.24284841
AGGGGCG38350.07.2338131
AGGGCGG39750.07.0925171
TGGGCGG45650.07.06517271
CGACGCT5156.982302E-66.987506442
AGCGTCG5552.7431288E-66.90849541
AGGCCCG33100.06.81395861
AGGCGAC15600.06.7951771
AGGCCGG67150.06.78473421
AGGGGGC77850.06.77929831
AGGCGCC60850.06.70881031
AGGGCGC24900.06.70285651
Produced by FastQC (version 0.11.5)