FastQCFastQC Report
Thu 8 Feb 2018
OD17_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD17_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21930861
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG5850.021.5734866
ATACGGC8700.014.5063095
ACGGCGA10000.012.8458557
TAGCCGG60900.012.4456971
CGACGCT7700.012.26717542
TGATACG10850.011.8394983
TACACGA9750.011.53379438
ACGACGC8850.011.43550241
TGGCCGG60950.011.2881641
CACGACG9500.011.12646740
GATACGG11450.011.0222614
TCCCGTT38650.010.447221
TGGGGCG43300.010.2630281
TGGGGTT148250.010.1339031
TAGCCGA8750.010.0543441
CCAACGA35550.09.8669489
TGGCCAG267650.09.827171
TCGTATA14050.09.76308312
GGGGTTT172850.09.6743692
AGGGCGG53850.09.5509441
Produced by FastQC (version 0.11.5)