FastQCFastQC Report
Thu 8 Feb 2018
OD18_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD18_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57677765
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG161000.013.0590341
TGGGCCG98300.010.0868241
TAGCCAG427300.010.0254651
TGGGGTT386200.09.9603951
TGGGGCG106250.09.9471631
AGGGGCG118300.09.6768561
TGGCCGG141250.09.6361551
AGGGTTT364200.09.6215661
AGGGCCG105200.09.6180251
AGGGCGG120500.09.5188841
TGGCCAG677850.09.3084991
TGGCGGC119650.09.1909921
TAGGCCG43650.09.13785651
AGGCCGG194100.08.8932091
TAGCCGA26950.08.7798241
TGGGGCC319600.08.7361441
TGGCGAA60100.08.6989891
GGGGTTT443300.08.60376552
TAGGCGC25700.08.5930671
AGGGGCC260950.08.5579881
Produced by FastQC (version 0.11.5)