FastQCFastQC Report
Thu 8 Feb 2018
OD19_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD19_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38969390
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG124550.012.0603911
TGGGCCG82150.010.5976761
TAGCCGA17500.010.1816921
TGGGGTT269000.010.1536641
TGGGGCG79150.09.7170491
TAGCCAG311100.09.7003411
AGGGCCG82050.09.6484921
TAGGCGG22200.09.346861
GGGGTTT318300.09.3200882
AGGGTTT241850.09.2231891
TAGGCCG30050.09.1568531
AGGGGCG93650.09.0073011
AGGGCGG97900.08.9388151
AGGCGCC130050.08.9315691
TGGCCAG479150.08.8965421
TGGCGAA44800.08.8606491
TGGCAAA216300.08.7902651
TGGCGAC30550.08.7855031
TGGGGCC235550.08.7708831
AGGCGGC96050.08.6883091