FastQCFastQC Report
Thu 8 Feb 2018
OD1_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD1_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36673710
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT18950.015.31790242
CGTATGC19350.014.299540544
GATCGGA19500.014.1146511
TCTCGTA20350.013.60064241
TCGTATG21300.013.4128843
ATCGGAA23600.011.8411232
TAGCCGG90050.011.2488351
ATCTCGT27800.010.44151940
TGGGCCG63950.010.2659081
ACGGGGT101300.010.2222891
ACACGTC31450.09.87086413
TAGGCCG25300.09.7196571
AGGGCGG72450.09.6842711
TGGCCGG84800.09.3912571
AGGGGCG70300.09.3386171
TCCCGTT64350.09.2555091
TGGGGTT230100.09.1868771
AGGCGCC107450.09.0913091
AGGGTTT215150.08.9234571
AGGGCCG60400.08.8896551
Produced by FastQC (version 0.11.5)