FastQCFastQC Report
Thu 8 Feb 2018
OD20_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD20_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55517337
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG133850.011.8970861
TGGGCCG130850.011.4458661
TAGGCCG46550.011.1930021
AGGGGCG160900.010.7801441
TGGCCGG164050.010.6281461
TGGGGTT377500.010.5936591
AGGGCCG143900.010.5802821
TGGGGCG135550.010.4832341
AGGGCGG164500.010.407111
TGGGGCC363350.010.2178351
TGGCGGC162650.010.0539841
TGGGCGG164900.010.0125491
AGGGTTT336100.09.9322461
AGGCCGG237150.09.872531
TGGCCCG106500.09.8270391
AGGGGGG142350.09.8240021
TGGCCAG658200.09.7528451
AGGCGCC216900.09.7231351
AGGCGGC168950.09.5589271
AGGGGCC303300.09.474391
Produced by FastQC (version 0.11.5)