FastQCFastQC Report
Thu 8 Feb 2018
OD2_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD2_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37133139
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18450.013.3126841
CTCGTAT18650.013.14903142
CGTATGC19600.012.74127944
TCTCGTA21050.011.97049341
TCGTATG21500.011.61530643
ATCGGAA24000.010.4069052
CGATCAG23800.010.30375833
TCCCGTT62050.09.9868021
TAGCCGG92550.09.7147581
AGGGGCG65900.09.3349671
ATCTCGT27800.09.22584540
GTCACGA26450.09.1866129
TGGGGTT232150.09.07566451
TGGGGCG58000.08.8581361
ACGATCA27850.08.80536632
TCCAACG59500.08.7723748
TCACGAT30350.08.67330130
CCAACGA59650.08.4485799
TAGGCCG22950.08.4440591
CAACGAA62000.08.38236210
Produced by FastQC (version 0.11.5)