FastQCFastQC Report
Thu 8 Feb 2018
OD3_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD3_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48432804
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26600.018.18379642
CGTATGC27900.017.57842644
TCGTATG29200.016.48764643
TCTCGTA30400.016.05884641
GATCGGA30950.015.962931
TATCTCG34100.014.97605439
ATCGGAA36000.013.9385762
ATCTCGT41050.012.00215240
ACACGTC47200.010.81955413
TAGCCGG127550.010.099151
ACGGGGT144750.09.9589021
AGGGCGG143400.09.7537521
TGGGCCG115100.09.6627511
AGGGGCG142200.09.4235821
CGTCTGA54000.09.4154316
AGGGCCG122350.09.237681
AGGCCGG206250.09.1222351
TGGGGCG122300.09.1123351
TGGCGGC146900.09.0760081
GTATGCC58000.08.99886445
Produced by FastQC (version 0.11.5)