FastQCFastQC Report
Thu 8 Feb 2018
OD4_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD4_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25438291
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA16050.013.8772532
CTCGTAT14050.013.77099742
CGTATGC16000.012.23326944
TCGTATG16650.012.02594143
GATCGGA17350.011.8191361
TCTCGTA17350.011.41107341
ATCGGAA20300.010.3089972
ATCTCGT21400.09.46176140
ACACGTC24250.09.27751213
TAGCCGG64550.08.8321841
CGGCTAC27550.08.8195333
TGGGGTT161500.08.5811671
TGGCGGA44900.08.1304111
AGGGGCG90400.08.1263021
CGGCTAA57200.08.10453320
TGGGCCG66800.08.096151
TAGGCCG20600.08.0948411
AGGGCCG70800.07.9570221
AGGGCGG91350.07.89378361
AGGGGGC128500.07.8913841
Produced by FastQC (version 0.11.5)