FastQCFastQC Report
Thu 8 Feb 2018
OD5_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD5_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31112296
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA15800.013.7098271
CTCGTAT15350.013.48378642
ATCGGAA18500.011.798952
TAGCCGG113500.011.7293511
TCTCGTA17850.011.46930241
CGTATGC18450.011.34015644
TCGTATG18350.011.27935243
ACGGGGT98250.010.150971
TGGGGTT214050.09.0446111
AGGGGCG58750.08.6415661
AGGGTTT192400.08.5846751
TGGCCGG83750.08.5137351
AGGGCCG54900.08.5077721
ATCTCGT24950.08.47600340
AGGGCGG64700.08.4048621
TGGCCAG393550.08.2734041
TACTCGG129450.08.25510437
CGGGGTT108000.08.2511572
ACACGTC28150.08.23167913
TAGGCCG24450.08.2135131
Produced by FastQC (version 0.11.5)