FastQCFastQC Report
Thu 8 Feb 2018
OD6_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD6_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71740118
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG224100.013.1733711
TGGGGTT454050.09.9661521
TAGCCAG601000.09.4304191
TAGCCCG25500.09.2793291
TGGCCAG848250.09.1550111
GGGGTTT559150.08.9937122
AGGGTTT452850.08.9624521
AGGCGCC149900.08.8247811
TGGCGAA69500.08.7872291
CGGGCGT131450.08.6814175
TGGCCGG133400.08.4803811
AGCCGGG344100.08.2304532
GTAGTCC463400.08.207026526
TACTCGG281100.08.1029137
TAGGCCG43750.08.0355211
CTACTCG294200.08.0177936
TGGCAAA424750.07.9053371
TAGCCGA30950.07.8637661
CGCCTGT462500.07.83450821
AGGGCCG81450.07.8023511