FastQCFastQC Report
Thu 8 Feb 2018
OD7_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD7_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89164061
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG236300.012.1112571
TAGGCCG74000.09.8664861
TGGGGTT570500.09.6379221
TGGGCCG202050.09.5692451
AGGGCGG269600.09.2779561
TGGGGCG219900.09.263871
AGGGGCG260350.09.2267521
AGGGCCG220300.09.0936141
AGGGTTT532350.09.0459981
TAGCCAG592900.08.8861221
TGGCGAA98400.08.7252751
AGGCGCC308300.08.6907571
TGGCCGG251250.08.6640371
TGGCGGC267750.08.6603441
AGGCCGG357450.08.5674861
TGGCCAG1009100.08.5529081
AGGGGGC414800.08.5455341
GGGGTTT682200.08.5240122
AGGGGCC461650.08.493481
AGGCGGC278100.08.410961