FastQCFastQC Report
Thu 8 Feb 2018
OD8_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD8_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26931226
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG65500.013.5209341
AGGGGCG43950.010.1009641
TAGCGCG3401.3695717E-79.9418641
TGGGCCG35400.09.7396561
TAGCCAG193500.09.5729621
TGGGGTT172650.09.3976961
TAGCCCG10350.09.3623231
CGGCTAA45850.09.16594120
TGGGGCG38150.09.1557221
TGGCCAG296450.09.1066951
AGGGCCG40250.09.0139571
TGGCCGG54800.09.0057331
TGGCGGC41900.08.8741221
TACTCGG84000.08.80789937
GGGGTTT202050.08.75420952
TGGCGTT17300.08.72738551
AGGCGAC16550.08.7143991
TAGGGCG9600.08.6853231
AGGCGCC65150.08.647311
AGGGTTT164550.08.6414561
Produced by FastQC (version 0.11.5)