FastQCFastQC Report
Thu 8 Feb 2018
OD9_k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD9_k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49199211
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG154250.013.1396841
TGGGGTT333900.010.9180221
TAGGCCG34300.010.7138091
TGGGCCG80600.09.7340731
TAGCCAG414500.09.5238381
TAGCCCG22300.09.5032771
TAGCCGA21500.09.4374461
AGGGTTT317000.09.3167081
GGGGTTT400300.09.2536292
AGGGGCG89400.09.22983551
TGGGGCG78300.09.1850031
TGGCCAG604400.09.0642581
AGGGCGG92150.09.0033241
TGGCGAA50950.08.9826041
TAGGCGT24750.08.7447311
AGGGGGG102150.08.7399161
TGGCCGG114300.08.6787431
TACTCGG189000.08.43473437
AGGGGGC184700.08.4345431
AGGCCGG159300.08.3783081
Produced by FastQC (version 0.11.5)